| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.128424 |
| Chromosome: | chromosome 16 |
| Location: | 6433209 |
| Confidence (%): | 58 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre16.g673553 | (1 of 1) PTHR10992//PTHR10992:SF906 - ALPHA/BETA HYDROLASE FOLD-CONTAINING PROTEIN // SUBFAMILY NOT NAMED | 5'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ATGAACATCTCCAGCAGCGACCAATTAGAT |
| Internal bar code: | GAATCCCCCCTCCTGGGAGGCT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 691 |
| LEAP-Seq percent confirming: | 99.7747 |
| LEAP-Seq n confirming: | 3100 |
| LEAP-Seq n nonconfirming: | 7 |
| LEAP-Seq n unique pos: | 22 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AAGAGAGCAGGAGAGAGGGG |
| Suggested primer 2: | TCATCGTCAAGACCTCCCTC |