| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.128491 |
| Chromosome: | chromosome 15 |
| Location: | 530192 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre15.g637050 | FAP274 | Flagellar Associated Protein | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | AGAACCACGACCAGCACTCACGCCCCTCGT |
| Internal bar code: | ACACGCTGACTGGGTCGAAAAC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 911 |
| LEAP-Seq percent confirming: | 99.7135 |
| LEAP-Seq n confirming: | 7656 |
| LEAP-Seq n nonconfirming: | 22 |
| LEAP-Seq n unique pos: | 8 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CCTCAGCTTACCTTCGTTCG |
| Suggested primer 2: | GAGAGAGTGCGTGTGCATGT |