Insertion junction: LMJ.RY0402.128494_1

Overview

Insertion cassette:CIB1
Side of cassette:5'
Strand:-
Strain:LMJ.RY0402.128494
Chromosome:chromosome 7
Location:1445261
Confidence (%):73
Locus systematic id Locus common name Defline Feature
Cre07.g323250 intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): GGGTGCGCAGAAGGTGTGGACGTGAGGGGC
Internal bar code:TCGATGATCTGAATATCAGCAG

Confirmation - LEAP-Seq

LEAP-Seq distance:904
LEAP-Seq percent confirming:81.25
LEAP-Seq n confirming:39
LEAP-Seq n nonconfirming:9
LEAP-Seq n unique pos:2

Suggested primers for confirmation by PCR

Suggested primer 1:CGCTCATCTCCTTTCCAGTC
Suggested primer 2:GAATCAACGACTTCGCTTCC