Insertion cassette: | CIB1 |
Side of cassette: | 5' |
Strand: | + |
Strain: | LMJ.RY0402.128502 |
Chromosome: | chromosome_14 |
Location: | 925833 |
Confidence (%): | 95 |
Locus systematic id | Locus common name | Defline | Orientation | Feature |
---|---|---|---|---|
Cre14.g613700 | antisense | CDS |
Insertion site details | |
Flanking sequence (orientation from cassette outwards): | GTCACAGCAACCATGTGCGTCATGGCAGCT |
Internal bar code: | TAAAGGCTGCTGCCGAGTTACT |
Confirmation - LEAP-Seq | |
LEAP-Seq distance: | 847 |
LEAP-Seq percent confirming: | 92.9538 |
LEAP-Seq n confirming: | 1649 |
LEAP-Seq n nonconfirming: | 125 |
LEAP-Seq n unique pos: | 2 |
Suggested primers for confirmation by PCR | |
Suggested primer 1: | CCCACCTCAAAGTCACCAGT |
Suggested primer 2: | TCTGATTCTGTTGGACAGCG |