| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.128503 |
| Chromosome: | chromosome 16 |
| Location: | 579163 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre16.g692350 | (1 of 10) PTHR23257//PTHR23257:SF520 - SERINE-THREONINE PROTEIN KINASE // IP11267P | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CATATGCCATGTCATCCGCACACAGCGGCC |
| Internal bar code: | GGTTTGCCAGATAGGGGTCCGT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 384 |
| LEAP-Seq percent confirming: | 81.6812 |
| LEAP-Seq n confirming: | 3848 |
| LEAP-Seq n nonconfirming: | 863 |
| LEAP-Seq n unique pos: | 9 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ATGCTGTAATGAAGGCCTGG |
| Suggested primer 2: | TGGCGGAATCCTACTATTGG |