Insertion junction: LMJ.RY0402.128508_1

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:-
Strain:LMJ.RY0402.128508
Chromosome:chromosome 14
Location:3244277
Confidence (%):73
Locus systematic id Locus common name Defline Feature
Cre14.g629300 (1 of 3) K11855 - ubiquitin carboxyl-terminal hydrolase 36/42 [EC:3.4.19.12] (USP36_42) gene_edge/mRNA_edge/3'UTR

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): GGTAATCGACAGTTACAGGCGCTGTGCGAA
Internal bar code:CGATGGTTACGGGATGGGTGGT

Confirmation - LEAP-Seq

LEAP-Seq distance:439
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:1525
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:4

Suggested primers for confirmation by PCR

Suggested primer 1:CCACACAAGTGCATTTGACC
Suggested primer 2:GAATATGTTCCAGAAGGCGG