Insertion cassette: | CIB1 |
Side of cassette: | 3' |
Strand: | + |
Strain: | LMJ.RY0402.128565 |
Chromosome: | chromosome 13 |
Location: | 2656038 |
Confidence (%): | 73 |
Locus systematic id | Locus common name | Defline | Feature |
---|---|---|---|
Cre13.g581700 | (1 of 6) 2.7.7.84 - 2'-5' oligoadenylate synthase | 5'UTR |
Insertion site details | |
Expected flanking sequence (starting at cassette; orientation from cassette outwards): | AGCTTGGGGTGTCCCTTGCTTCCAGCGCCA |
Internal bar code: | AAGCGGTACAGGTGGAAAATGA |
Confirmation - LEAP-Seq | |
LEAP-Seq distance: | 277 |
LEAP-Seq percent confirming: | 90.6615 |
LEAP-Seq n confirming: | 233 |
LEAP-Seq n nonconfirming: | 24 |
LEAP-Seq n unique pos: | 1 |
Suggested primers for confirmation by PCR | |
Suggested primer 1: | TCGTCAGGCACAGTCTCATC |
Suggested primer 2: | AGGCATGCGATCCACTAAAC |