| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.128568 |
| Chromosome: | chromosome 16 |
| Location: | 4139623 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre16.g669125 | ADH13B | Dual function alcohol/acetaldehyde dehydrogenase, pseudogene 2 | 5'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TGAACAGCCGCGACTACCGCGGGCTGAGGC |
| Internal bar code: | ATTTGTGCAGCCGCTGCAGCCT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 417 |
| LEAP-Seq percent confirming: | 76.4645 |
| LEAP-Seq n confirming: | 49250 |
| LEAP-Seq n nonconfirming: | 15159 |
| LEAP-Seq n unique pos: | 75 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CGCACCTTCATTCACTGCTA |
| Suggested primer 2: | TGCTCAGTCTGTTCACCTGG |