Insertion junction: LMJ.RY0402.128572_2

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:-
Strain:LMJ.RY0402.128572
Chromosome:chromosome 17
Location:4173856
Confidence (%):73
Locus systematic id Locus common name Defline Feature
Cre17.g730000 intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): GAAACAGCTGAACGTGGCGCCACCAGCACA
Internal bar code:TGACCAGCCAGGACGGGAGACG

Confirmation - LEAP-Seq

LEAP-Seq distance:690
LEAP-Seq percent confirming:84.3293
LEAP-Seq n confirming:635
LEAP-Seq n nonconfirming:118
LEAP-Seq n unique pos:4

Suggested primers for confirmation by PCR

Suggested primer 1:CATACACACACGAACAGCCC
Suggested primer 2:ACAACGAAACGGAACAAAGG