| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.128574 |
| Chromosome: | chromosome 3 |
| Location: | 697625 |
| Confidence (%): | 58 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre03.g145987 | (1 of 7) IPR000104//IPR020683 - Antifreeze protein, type I // Ankyrin repeat-containing domain | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CGATAGCAGCCGCAGCGGCAGTCGCTCCGC |
| Internal bar code: | CCGACGTCGCGGCAGCGGGCAT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 225 |
| LEAP-Seq percent confirming: | 88.9279 |
| LEAP-Seq n confirming: | 2530 |
| LEAP-Seq n nonconfirming: | 315 |
| LEAP-Seq n unique pos: | 9 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CTACTGCCTCTCCTGAACGG |
| Suggested primer 2: | CCTCACACCAAAGCCAAAGT |