Insertion junction: LMJ.RY0402.128592_2

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:-
Strain:LMJ.RY0402.128592
Chromosome:chromosome 6
Location:3151292
Confidence (%):95
Locus systematic id Locus common name Defline Feature
Cre06.g275400 (1 of 1) PTHR10331 - T COMPLEX PROTEIN 10 intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): TCCCAACAATCGCAACCACGACCTCATAAA
Internal bar code:TGATTACTCCGAGGTGTTGCGC

Confirmation - LEAP-Seq

LEAP-Seq distance:79
LEAP-Seq percent confirming:97.1867
LEAP-Seq n confirming:760
LEAP-Seq n nonconfirming:22
LEAP-Seq n unique pos:7

Suggested primers for confirmation by PCR

Suggested primer 1:ACAGCAATACACGCAACAGC
Suggested primer 2:TACAGCTCGTCGTCACCAAG