Insertion cassette: | CIB1 |
Side of cassette: | 3' |
Strand: | + |
Strain: | LMJ.RY0402.128592 |
Chromosome: | chromosome_11 |
Location: | 327321 |
Confidence (%): | 73 |
Locus systematic id | Locus common name | Defline | Orientation | Feature |
---|---|---|---|---|
Cre11.g467567 | UBQ1 | Ubiquitin | antisense | intron |
Insertion site details | |
Flanking sequence (orientation from cassette outwards): | TGAGAGGAAACGCTCGTGTCAGACAAAGTG |
Internal bar code: | GACTGATCGGGAGCGCATATCG |
Confirmation - LEAP-Seq | |
LEAP-Seq distance: | 893 |
LEAP-Seq percent confirming: | 90.7121 |
LEAP-Seq n confirming: | 293 |
LEAP-Seq n nonconfirming: | 30 |
LEAP-Seq n unique pos: | 4 |
Suggested primers for confirmation by PCR | |
Suggested primer 1: | CCCTCTGTAACATCCGCATT |
Suggested primer 2: | ACCATTGAGAACGTGAAGGC |