| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.128609 |
| Chromosome: | chromosome 6 |
| Location: | 3299432 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre06.g277200 | BIOA,BIO1 | Diaminopelargonic acid synthase; (1 of 1) 6.3.3.3 - Dethiobiotin synthase / DTB synthetase | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TGCTGCCCCGGCTCCGCCGCGCCGCCGCAC |
| Internal bar code: | TACGGTGGGTCGTCGCTAGGGC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 133 |
| LEAP-Seq percent confirming: | 89.317 |
| LEAP-Seq n confirming: | 1020 |
| LEAP-Seq n nonconfirming: | 122 |
| LEAP-Seq n unique pos: | 6 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TACTCAAAACACACACGCCC |
| Suggested primer 2: | CTCTCGCTTGCCTCCATTAC |