| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.128615 |
| Chromosome: | chromosome 7 |
| Location: | 6334730 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre07.g357200 | UGD1,EZY12 | Similar to UDP-Glucose Dehydrogenase; (1 of 3) 1.1.1.22 - UDP-glucose 6-dehydrogenase | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TGAACGCAACATACTCAGGGCCCCCGGCCC |
| Internal bar code: | GCTGTCGTCTGGCCATGTTGAC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 421 |
| LEAP-Seq percent confirming: | 99.208 |
| LEAP-Seq n confirming: | 10898 |
| LEAP-Seq n nonconfirming: | 87 |
| LEAP-Seq n unique pos: | 4 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GTAACGGTGAGGTTAGGCGA |
| Suggested primer 2: | ATGTAACGCCGCCTGTTTAG |