| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.128622 |
| Chromosome: | chromosome 2 |
| Location: | 6820306 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre02.g119050 | CER1 | Carboxyesterase-related protein; (1 of 2) K15889 - prenylcysteine alpha-carboxyl methylesterase (PCME) | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TCCAGCAGCTGTATGAACTAGTCAGGCGGG |
| Internal bar code: | GTTGCGCTTACGTGAGGCTCTG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 829 |
| LEAP-Seq percent confirming: | 95.8255 |
| LEAP-Seq n confirming: | 1538 |
| LEAP-Seq n nonconfirming: | 67 |
| LEAP-Seq n unique pos: | 16 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AGCCAACACAATGTGGATGA |
| Suggested primer 2: | TACGCCAACGAGTAGTGTCG |