Insertion junction: LMJ.RY0402.128676_1

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:-
Strain:LMJ.RY0402.128676
Chromosome:chromosome 14
Location:525424
Confidence (%):95
Locus systematic id Locus common name Defline Feature
Cre14.g611484 (1 of 4) IPR023485 - Phosphotyrosine protein phosphatase I superfamily 3'UTR

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): TCCTGCAGCTGCCGACAGCGACTTGATTCG
Internal bar code:GGGGGTCTGCGATCGACGCGAC

Confirmation - LEAP-Seq

LEAP-Seq distance:928
LEAP-Seq percent confirming:76.2726
LEAP-Seq n confirming:929
LEAP-Seq n nonconfirming:289
LEAP-Seq n unique pos:14

Suggested primers for confirmation by PCR

Suggested primer 1:ATTGATTGGATCTGCAAGGG
Suggested primer 2:TCCTGATGTAGCGTCTGGTG