Insertion junction: LMJ.RY0402.128676_2

Overview

Insertion cassette:CIB1
Side of cassette:5'
Strand:-
Strain:LMJ.RY0402.128676
Chromosome:chromosome 14
Location:525571
Confidence (%):95
Locus systematic id Locus common name Defline Feature
Cre14.g611484 (1 of 4) IPR023485 - Phosphotyrosine protein phosphatase I superfamily 3'UTR

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CAGGAAGTGTCATTACTGCCATTACAGAAC
Internal bar code:CTGTGAATGGGAGGTCCCTCAT

Confirmation - LEAP-Seq

LEAP-Seq distance:0
LEAP-Seq percent confirming:99.6032
LEAP-Seq n confirming:251
LEAP-Seq n nonconfirming:1
LEAP-Seq n unique pos:1

Suggested primers for confirmation by PCR

Suggested primer 1:GATGATGATGACACGCTTGG
Suggested primer 2:TCCTGATGTAGCGTCTGGTG