| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.128681 |
| Chromosome: | chromosome 6 |
| Location: | 2307008 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre06.g267100 | SRP68 | (1 of 1) K03107 - signal recognition particle subunit SRP68 (SRP68); Subunit of the Signal Recognition Particle | 5'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GCTGAAAGGGTCCGATCGCCCGTGGTCGGC |
| Internal bar code: | GCCACGACCATTGAGTCCTTGG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 594 |
| LEAP-Seq percent confirming: | 99.3559 |
| LEAP-Seq n confirming: | 617 |
| LEAP-Seq n nonconfirming: | 4 |
| LEAP-Seq n unique pos: | 2 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CTTGTTTGCTTTCTGGAGCC |
| Suggested primer 2: | CACCCCGTGCTTCTGTACTT |