Insertion junction: LMJ.RY0402.128691_2

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:-
Strain:LMJ.RY0402.128691
Chromosome:chromosome 15
Location:542903
Confidence (%):58
Locus systematic id Locus common name Defline Feature
intergenic

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): AAATCGCCCAACCTGAGACCGCGTCTACCA
Internal bar code:GTTTGCCAAGGCAGCTCGGTTT

Confirmation - LEAP-Seq

LEAP-Seq distance:215
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:65
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:3

Suggested primers for confirmation by PCR

Suggested primer 1:TTACCTTGTTGACGTGCGAG
Suggested primer 2:CCAGAAAAGCCAGCTACGTC