| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.128693 |
| Chromosome: | chromosome 2 |
| Location: | 6930034 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre02.g119950 | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CTTAACCACGCCTGCAAGCCCCCGCAACAC |
| Internal bar code: | GTGTATGCCGCGGAAATATCGG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 942 |
| LEAP-Seq percent confirming: | 78.087 |
| LEAP-Seq n confirming: | 449 |
| LEAP-Seq n nonconfirming: | 126 |
| LEAP-Seq n unique pos: | 10 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TGGTCACAACATCACACACG |
| Suggested primer 2: | GCTACTGTGCGTAGCTGCTG |