| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.128699 |
| Chromosome: | chromosome 9 |
| Location: | 2263133 |
| Confidence (%): | 58 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre09.g392652 | (1 of 1) 1.5.1.22 - Strombine dehydrogenase | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ATCAGTAACAGCCGCCGCAGCAGCTGTTGC |
| Internal bar code: | TAATTAGGGCGCGGCTGTCAAA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 433 |
| LEAP-Seq percent confirming: | 43.9319 |
| LEAP-Seq n confirming: | 4181 |
| LEAP-Seq n nonconfirming: | 5336 |
| LEAP-Seq n unique pos: | 20 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TATTCTGCTGCATGCTGGTC |
| Suggested primer 2: | AGACCCCAATCAAGCAACCT |