Insertion junction: LMJ.RY0402.128801_2

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:-
Strain:LMJ.RY0402.128801
Chromosome:chromosome 14
Location:526869
Confidence (%):73
Locus systematic id Locus common name Defline Feature
Cre14.g611484 (1 of 4) IPR023485 - Phosphotyrosine protein phosphatase I superfamily 3'UTR

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CATTTGTCCCGCCCGGTCGCCATGGGGTCG
Internal bar code:ATGTACGGCTAGTAGGGGGGCG

Confirmation - LEAP-Seq

LEAP-Seq distance:731
LEAP-Seq percent confirming:98.9796
LEAP-Seq n confirming:291
LEAP-Seq n nonconfirming:3
LEAP-Seq n unique pos:2

Suggested primers for confirmation by PCR

Suggested primer 1:GGAATGAAATACCCCCTGCT
Suggested primer 2:CCAAGCGTGTCATCATCATC