Insertion junction: LMJ.RY0402.128847_1

Overview

Insertion cassette:CIB1
Side of cassette:5'
Strand:+
Strain:LMJ.RY0402.128847
Chromosome:chromosome 4
Location:2056064
Confidence (%):95
Locus systematic id Locus common name Defline Feature
Cre04.g219050 SRR10 Scavenger receptor cysteine rich (SRCR) protein CDS

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): TGTACGTCTTTGTACGACAGCCGGGCGGTA
Internal bar code:GCAGTGGTTCGTCATCTTTGTA

Confirmation - LEAP-Seq

LEAP-Seq distance:764
LEAP-Seq percent confirming:99.522
LEAP-Seq n confirming:5621
LEAP-Seq n nonconfirming:27
LEAP-Seq n unique pos:4

Suggested primers for confirmation by PCR

Suggested primer 1:TTTAGAACCTCTCCCCACCA
Suggested primer 2:CCGTAACCAGACAAAGCCAT