| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.128884 |
| Chromosome: | chromosome 10 |
| Location: | 768819 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre10.g422900 | (1 of 10) PTHR23257:SF520 - IP11267P | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GTTGCAAGCACCGCATCCGTACCCATGGCG |
| Internal bar code: | CTTCGATCCTTGACCGGTGGAC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 839 |
| LEAP-Seq percent confirming: | 88.8504 |
| LEAP-Seq n confirming: | 7188 |
| LEAP-Seq n nonconfirming: | 902 |
| LEAP-Seq n unique pos: | 27 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ATGTAGCACGTGCGTCGTAG |
| Suggested primer 2: | CACCGTTAAATGTGCAGTGG |