| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.128891 |
| Chromosome: | chromosome 12 |
| Location: | 4152893 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre12.g518200 | PDI1,DNJ3 | (1 of 1) PTHR18929//PTHR18929:SF103//PTHR24078//PTHR24078:SF252 - PROTEIN DISULFIDE ISOMERASE // PROTEIN DISULFIDE-ISOMERASE A5 // DNAJ HOMOLOG SUBFAMILY C MEMBER // SUBFAMILY NOT NAMED; Protein disulfide isomerase, DnaJ-like protein | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CCAGGTCCGTGCCGGCGTCCAGCCGCACCT |
| Internal bar code: | GTGTCCCCTCAGGCGGGTCGGA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 564 |
| LEAP-Seq percent confirming: | 67.3375 |
| LEAP-Seq n confirming: | 5086 |
| LEAP-Seq n nonconfirming: | 2467 |
| LEAP-Seq n unique pos: | 26 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TTGGCATCAGCAGAGTGAAC |
| Suggested primer 2: | GGTCGTGGGTCTGAGTGATT |