Insertion junction: LMJ.RY0402.128897_2

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:+
Strain:LMJ.RY0402.128897
Chromosome:chromosome 8
Location:3966732
Confidence (%):95
Locus systematic id Locus common name Defline Feature
Cre08.g378750 CTAP7 Chloroplast translocon associated protein 7 3'UTR

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): TTGTGTGGGTCTGCCTGTGGGGCGGGCAAC
Internal bar code:GGCGTCTCACCCAGACCAGGAG

Confirmation - LEAP-Seq

LEAP-Seq distance:571
LEAP-Seq percent confirming:99.1899
LEAP-Seq n confirming:2816
LEAP-Seq n nonconfirming:23
LEAP-Seq n unique pos:17

Suggested primers for confirmation by PCR

Suggested primer 1:AAGATTACTGGGGCGGAGAT
Suggested primer 2:GAAGTACCCAGCGAGTGAGC