| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.128904 |
| Chromosome: | chromosome 15 |
| Location: | 497290 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre15.g636750 | GST11 | (1 of 15) 2.5.1.18 - Glutathione transferase / S-(hydroxyalkyl)glutathione lyase; Glutathione S-transferase | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TCCCCGCCGTTTCCCCCCTTCCCGTCCGCC |
| Internal bar code: | ATCATCATGTGTGAGTATCCCA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 286 |
| LEAP-Seq percent confirming: | 98.5053 |
| LEAP-Seq n confirming: | 2702 |
| LEAP-Seq n nonconfirming: | 41 |
| LEAP-Seq n unique pos: | 2 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GCCGAAACGGGTAAACACTA |
| Suggested primer 2: | CACACAAACCCAGGAGGACT |