Insertion cassette: | CIB1 |
Side of cassette: | 3' |
Strand: | - |
Strain: | LMJ.RY0402.128928 |
Chromosome: | chromosome 12 |
Location: | 7782977 |
Confidence (%): | 73 |
Locus systematic id | Locus common name | Defline | Feature |
---|---|---|---|
Cre12.g553600 | (1 of 68) 2.1.1.43 - Histone-lysine N-methyltransferase / Protein-lysine N-methyltransferase | intron |
Insertion site details | |
Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CTTGACCGCACGGGGCCGCAGGCGGCGAGC |
Internal bar code: | TGGGCTGTCCATTGGTTGACAC |
Confirmation - LEAP-Seq | |
LEAP-Seq distance: | 61 |
LEAP-Seq percent confirming: | 67.9389 |
LEAP-Seq n confirming: | 89 |
LEAP-Seq n nonconfirming: | 42 |
LEAP-Seq n unique pos: | 3 |
Suggested primers for confirmation by PCR | |
Suggested primer 1: | TTCTGGGCGTGTGTATGTGT |
Suggested primer 2: | GTGCATAGTTTGGGGCAGTT |