| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.128969 |
| Chromosome: | chromosome 4 |
| Location: | 2056204 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre04.g219050 | SRR10 | Scavenger receptor cysteine rich (SRCR) protein | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CACCCCGACGGACTATGACTTTGAGGCGGC |
| Internal bar code: | GGGGGTGAGCGATAAGATTTCA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 126 |
| LEAP-Seq percent confirming: | 99.5643 |
| LEAP-Seq n confirming: | 914 |
| LEAP-Seq n nonconfirming: | 4 |
| LEAP-Seq n unique pos: | 2 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TATCGTGCATACCCGACAGA |
| Suggested primer 2: | CCGTAACCAGACAAAGCCAT |