Insertion junction: LMJ.RY0402.128969_2

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:+
Strain:LMJ.RY0402.128969
Chromosome:chromosome 4
Location:2056206
Confidence (%):95
Locus systematic id Locus common name Defline Feature
Cre04.g219050 SRR10 Scavenger receptor cysteine rich (SRCR) protein CDS

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): GCTGATTTGGATGAGGCTGTACGAGTCTGT
Internal bar code:CCCCCGCCCCGCATGCATCCGG

Confirmation - LEAP-Seq

LEAP-Seq distance:762
LEAP-Seq percent confirming:99.7963
LEAP-Seq n confirming:490
LEAP-Seq n nonconfirming:1
LEAP-Seq n unique pos:7

Suggested primers for confirmation by PCR

Suggested primer 1:TATCGTGCATACCCGACAGA
Suggested primer 2:CCGTAACCAGACAAAGCCAT