| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.128982 |
| Chromosome: | chromosome 13 |
| Location: | 1716654 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre13.g574375 | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GCATTGATTGTGAGGACAACCATATCAATG |
| Internal bar code: | GTGAGGGGGGAGAGTAGAGAC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 979 |
| LEAP-Seq percent confirming: | 99.611 |
| LEAP-Seq n confirming: | 2817 |
| LEAP-Seq n nonconfirming: | 11 |
| LEAP-Seq n unique pos: | 14 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ACCGTACAGGCCATCTCAAC |
| Suggested primer 2: | TAAAGGAGCGGCAGGTTCTA |