| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.128983 |
| Chromosome: | chromosome 2 |
| Location: | 630274 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre02.g077951 | (1 of 14) 3.6.3.25 - Sulfate-transporting ATPase; putative ABC family transporter | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GCTGTGGGTCAACAACAGCGATGTCCTGGT |
| Internal bar code: | CTGGGAGCGACTAGCAGGAACT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 381 |
| LEAP-Seq percent confirming: | 91.2678 |
| LEAP-Seq n confirming: | 1735 |
| LEAP-Seq n nonconfirming: | 166 |
| LEAP-Seq n unique pos: | 8 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GCGAAACTGTTCCTGGCTAC |
| Suggested primer 2: | ACACACACACAAACGCACCT |