| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.129001 |
| Chromosome: | chromosome 1 |
| Location: | 6304478 |
| Confidence (%): | 58 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre01.g044900 | (1 of 5) PTHR10351:SF42 - LD40663P | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TGTTTAGTACATATATCAAGTATTTCATGG |
| Internal bar code: | GTACATACCCCCATACTAACGC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1409 |
| LEAP-Seq percent confirming: | 99.5381 |
| LEAP-Seq n confirming: | 431 |
| LEAP-Seq n nonconfirming: | 2 |
| LEAP-Seq n unique pos: | 2 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TCACTGCTGGAAGAGGAGGT |
| Suggested primer 2: | AAATGTGTGCTGTGTCGCTC |