| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.129022 |
| Chromosome: | chromosome 14 |
| Location: | 2344273 |
| Confidence (%): | 58 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre14.g623800 | NAT6 | N-acetyltransferase; (1 of 1) PTHR23091//PTHR23091:SF66 - N-TERMINAL ACETYLTRANSFERASE // N-ALPHA-ACETYLTRANSFERASE 30 | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CCACCACACTCGTCATTAACGAGCGTATCC |
| Internal bar code: | TTTCAGGTTGCACACGTGAGGG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 484 |
| LEAP-Seq percent confirming: | 99.1066 |
| LEAP-Seq n confirming: | 1553 |
| LEAP-Seq n nonconfirming: | 14 |
| LEAP-Seq n unique pos: | 11 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GACAAATGTGCGGGTCTTTT |
| Suggested primer 2: | CGCTGACCGAAGAGAAGAAG |