| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.129031 |
| Chromosome: | chromosome 6 |
| Location: | 3053839 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre06.g274450 | SMM20 | (1 of 5) PTHR13271//PTHR13271:SF28 - UNCHARACTERIZED PUTATIVE METHYLTRANSFERASE // SUBFAMILY NOT NAMED; S-adenosyl-L-methionine-dependent methyltransferase | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TAGCAGCGCAACTCCTACTGGCGCCCATAG |
| Internal bar code: | TCGTTAGGACATTGCAGCCCTG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 344 |
| LEAP-Seq percent confirming: | 93.1179 |
| LEAP-Seq n confirming: | 1556 |
| LEAP-Seq n nonconfirming: | 115 |
| LEAP-Seq n unique pos: | 10 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CAAATTTGCGCTACCTGACA |
| Suggested primer 2: | TTGACACTGTGTGTGTGCGT |