Insertion junction: LMJ.RY0402.129037_2

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:+
Strain:LMJ.RY0402.129037
Chromosome:chromosome 7
Location:3942450
Confidence (%):95
Locus systematic id Locus common name Defline Feature
Cre07.g339350 (1 of 1) K14050 - geranylgeranyl transferase type-2 subunit alpha [EC:2.5.1.60] (RABGGTA) 3'UTR

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): GGCATGCTACATGTAAGACCATGAGTTATC
Internal bar code:ACCCGGCTGTGGGACGAGGGTG

Confirmation - LEAP-Seq

LEAP-Seq distance:869
LEAP-Seq percent confirming:98.7321
LEAP-Seq n confirming:3037
LEAP-Seq n nonconfirming:39
LEAP-Seq n unique pos:19

Suggested primers for confirmation by PCR

Suggested primer 1:ATTCCCAACTGCTCCTGATG
Suggested primer 2:GACGTCGGTATCGGTGCTAT