| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.129069 |
| Chromosome: | chromosome 16 |
| Location: | 2552032 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre16.g661400 | (1 of 1) PTHR22979 - ZINC FINGER PROTEIN-RELATED | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CAGTAGGCACGCCTGCAGCCTGGCATTGCG |
| Internal bar code: | TTCGGGTAGGCGATGTTGGTAG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 489 |
| LEAP-Seq percent confirming: | 98.6866 |
| LEAP-Seq n confirming: | 6537 |
| LEAP-Seq n nonconfirming: | 87 |
| LEAP-Seq n unique pos: | 29 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GTGTAAGTTTGTCCCGCGAT |
| Suggested primer 2: | GGAGCAAGAGTTCAAGTCGC |