| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.129074 |
| Chromosome: | chromosome 10 |
| Location: | 5979119 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre10.g462500 | PUS16 | (1 of 1) 5.4.99.42 - tRNA pseudouridine(31) synthase; RNA pseudouridine synthase | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TTGAGGGCGGCAAAACTAAGGCGGCGTCGA |
| Internal bar code: | TAACCGTCTGGCCGATCCACCT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 731 |
| LEAP-Seq percent confirming: | 99.1379 |
| LEAP-Seq n confirming: | 4830 |
| LEAP-Seq n nonconfirming: | 42 |
| LEAP-Seq n unique pos: | 6 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TCATGACCTGCAAACTCAGC |
| Suggested primer 2: | CGGGTGTGTCTTTGTGTTTG |