| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.129108 |
| Chromosome: | chromosome 13 |
| Location: | 1855160 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre13.g575400 | (1 of 33) IPR013830 - SGNH hydrolase-type esterase domain | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CACGCATGAGGCACGCGACCACACGTGTTG |
| Internal bar code: | GTCTCAGTCGTCACGGAGCTCG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 582 |
| LEAP-Seq percent confirming: | 95.9564 |
| LEAP-Seq n confirming: | 1495 |
| LEAP-Seq n nonconfirming: | 63 |
| LEAP-Seq n unique pos: | 10 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CCCCTGATAACGCTTGTTGT |
| Suggested primer 2: | TAGTGTTCGTTTGCTGCTGG |