| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.129132 |
| Chromosome: | chromosome 17 |
| Location: | 2553332 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre17.g716600 | (1 of 752) IPR027417 - P-loop containing nucleoside triphosphate hydrolase | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TCGGGATCAGCAGCATGTTAGCAGCAGCCC |
| Internal bar code: | CTCAGGCTCGGCATTATAATTA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 544 |
| LEAP-Seq percent confirming: | 99.6795 |
| LEAP-Seq n confirming: | 6843 |
| LEAP-Seq n nonconfirming: | 22 |
| LEAP-Seq n unique pos: | 24 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GGACATCCTCCTGTTGCTGT |
| Suggested primer 2: | CGCCCCAAGCTGTACATAAT |