Insertion junction: LMJ.RY0402.129145_1

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:+
Strain:LMJ.RY0402.129145
Chromosome:chromosome 8
Location:3564627
Confidence (%):95
Locus systematic id Locus common name Defline Feature
Cre08.g375650 CNS1 (1 of 1) PTHR22904:SF389 - DNA POLYMERASE INTERACTING TPR CONTAINING PROTEIN OF 47KD; Cyclophilin seven suppressor 1 intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): GTTAGCTAACGAAATGGTAGACGGTAGGGG
Internal bar code:GGATTCGGGATGCGCTGTACGC

Confirmation - LEAP-Seq

LEAP-Seq distance:311
LEAP-Seq percent confirming:98.5507
LEAP-Seq n confirming:68
LEAP-Seq n nonconfirming:1
LEAP-Seq n unique pos:23

Suggested primers for confirmation by PCR

Suggested primer 1:CCTCCACCAGTTGTTCCTGT
Suggested primer 2:ATTGAGACGCATCCACATGA