Insertion cassette: | CIB1 |
Side of cassette: | 5' |
Strand: | + |
Strain: | LMJ.RY0402.129188 |
Chromosome: | chromosome 8 |
Location: | 789399 |
Confidence (%): | 95 |
Locus systematic id | Locus common name | Defline | Feature |
---|---|---|---|
intergenic |
Insertion site details | |
Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GCGGGTGGACACAGAGCGGGAGAGGAAGGG |
Internal bar code: | GCCGTTATGCACTGGCAACACG |
Confirmation - LEAP-Seq | |
LEAP-Seq distance: | 246 |
LEAP-Seq percent confirming: | 96.5147 |
LEAP-Seq n confirming: | 360 |
LEAP-Seq n nonconfirming: | 13 |
LEAP-Seq n unique pos: | 3 |
Suggested primers for confirmation by PCR | |
Suggested primer 1: | TGCCAACGCACATATCATCT |
Suggested primer 2: | TCCCTACGCTAACGGACCTA |