| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.129195 |
| Chromosome: | chromosome 12 |
| Location: | 4357667 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre12.g520200 | CPL8,PPO5 | (1 of 1) PTHR13343//PTHR13343:SF16 - CREG1 PROTEIN // SUBFAMILY NOT NAMED; Conserved in the Plant Lineage | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ACAGGACTGGTGTAAAGCAAGGCAGGTTCC |
| Internal bar code: | GCACGGCTTGTTCCCTCTACAC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 192 |
| LEAP-Seq percent confirming: | 99.5516 |
| LEAP-Seq n confirming: | 222 |
| LEAP-Seq n nonconfirming: | 1 |
| LEAP-Seq n unique pos: | 1 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TGAAGTTTGCGAGGTCAGTG |
| Suggested primer 2: | GCTTTCCTGACATCTGAGCC |