| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.129221 |
| Chromosome: | chromosome_2 |
| Location: | 5868541 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Orientation | Feature |
|---|---|---|---|---|
| None |
Insertion site details | |
| Flanking sequence (orientation from cassette outwards): | AGGGCTCTGAATCGCTCTGAAGGGCTCTGC |
| Internal bar code: | GAATCGTGGCGTGAGCACGCGG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 262 |
| LEAP-Seq percent confirming: | 49.5775 |
| LEAP-Seq n confirming: | 352 |
| LEAP-Seq n nonconfirming: | 358 |
| LEAP-Seq n unique pos: | 1 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ATGGCGAACCTATAGCCCTT |
| Suggested primer 2: | CCTGGAAGTTGTCATTCCGT |