Insertion junction: LMJ.RY0402.129221_3

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:+
Strain:LMJ.RY0402.129221
Chromosome:chromosome_14
Location:1379539
Confidence (%):73
Locus systematic id Locus common name Defline Orientation Feature
Cre14.g617250 FAP282 Flagellar Associated Protein sense CDS

Insertion site details

Flanking sequence (orientation from cassette outwards):TTCATCGAGCGCCTGATAACGGTAGGGGGC
Internal bar code:GTCGAGGTCGATACATGAGGTG

Confirmation - LEAP-Seq

LEAP-Seq distance:988
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:1319
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:6

Suggested primers for confirmation by PCR

Suggested primer 1:AGAACCCGTGGTGTTGTAGC
Suggested primer 2:CCATATGACTACGGCACACG