| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.129235 |
| Chromosome: | chromosome 1 |
| Location: | 3082005 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre01.g019100 | HPR2 | Hydroxypyruvate reductase 2; (1 of 1) PTHR10996//PTHR10996:SF20 - 2-HYDROXYACID DEHYDROGENASE-RELATED // D-3-PHOSPHOGLYCERATE DEHYDROGENASE | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TCGGACACCGCGCGGGCGCGTTGTGCATGT |
| Internal bar code: | TCCCTTGGTTCGTGGCTACGAA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 702 |
| LEAP-Seq percent confirming: | 99.7276 |
| LEAP-Seq n confirming: | 2929 |
| LEAP-Seq n nonconfirming: | 8 |
| LEAP-Seq n unique pos: | 2 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ATGGAAGGGAACAGCCTTCT |
| Suggested primer 2: | GGGTTGCTTGGCAATGTAGT |