| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.129239 |
| Chromosome: | chromosome_10 |
| Location: | 1318852 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Orientation | Feature |
|---|---|---|---|---|
| Cre10.g427600 | KAT1 | Katanin catalytic subunit, 60 kDa | sense | CDS |
Insertion site details | |
| Flanking sequence (orientation from cassette outwards): | CAAGATCAACCTGCGGGTGGGTGGCGGGCA |
| Internal bar code: | GCATGCGGTGATAACTAAGCGC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 190 |
| LEAP-Seq percent confirming: | 99.8483 |
| LEAP-Seq n confirming: | 1974 |
| LEAP-Seq n nonconfirming: | 3 |
| LEAP-Seq n unique pos: | 4 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CTTCTTCAACGTCTCCTCCG |
| Suggested primer 2: | AGCGAACTTGCAGCACCTAT |