Insertion junction: LMJ.RY0402.129244_2

Overview

Insertion cassette:CIB1
Side of cassette:5'
Strand:-
Strain:LMJ.RY0402.129244
Chromosome:chromosome 1
Location:2934182
Confidence (%):95
Locus systematic id Locus common name Defline Feature
Cre01.g017900 (1 of 8) IPR011047//IPR018391 - Quinonprotein alcohol dehydrogenase-like superfamily // Pyrrolo-quinoline quinone beta-propeller repeat intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CGGGCATACGTGCCGCCCGCCCGCTGCAGG
Internal bar code:CGGGATTAGCTGCCTTCTGTCC

Confirmation - LEAP-Seq

LEAP-Seq distance:106
LEAP-Seq percent confirming:99.2792
LEAP-Seq n confirming:1515
LEAP-Seq n nonconfirming:11
LEAP-Seq n unique pos:2

Suggested primers for confirmation by PCR

Suggested primer 1:TTCCGCTGACTTCTTCGTTT
Suggested primer 2:GGGTGCAGCAATACTTTGGT