| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.129263 |
| Chromosome: | chromosome 13 |
| Location: | 3741371 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre13.g589450 | FBB6L1 | (1 of 1) IPR002035//IPR013694 - von Willebrand factor, type A // VIT domain; FBB6 like protein 1 | 5'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TCCGGCGCAGCTGCCCGCGTGCTTTTTCGC |
| Internal bar code: | CAGTATAACTGGCGCGTACGCG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 411 |
| LEAP-Seq percent confirming: | 99.9547 |
| LEAP-Seq n confirming: | 4413 |
| LEAP-Seq n nonconfirming: | 2 |
| LEAP-Seq n unique pos: | 13 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AACATGCCTGGTTGTAAGCC |
| Suggested primer 2: | GAGGTAGCTGAACTCCACCG |