| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.129273 |
| Chromosome: | chromosome_3 |
| Location: | 7881849 |
| Confidence (%): | 58 |
| Locus systematic id | Locus common name | Defline | Orientation | Feature |
|---|---|---|---|---|
| Cre03.g202550 | ELG11 | Exostosin-like glycosyltransferase | sense | 5'UTR |
Insertion site details | |
| Flanking sequence (orientation from cassette outwards): | GGACCAAATCGCGCTAATTCCGGAAGTCTT |
| Internal bar code: | TTACTGTGGTAGGACCTGCGCG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 676 |
| LEAP-Seq percent confirming: | 98.4375 |
| LEAP-Seq n confirming: | 252 |
| LEAP-Seq n nonconfirming: | 4 |
| LEAP-Seq n unique pos: | 4 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ATCCTCCACTCACTGCTGCT |
| Suggested primer 2: | ATGAAGCATGCAGTCAGCAC |